protein 1 igfbp1 protein  (R&D Systems)

Journal: Bioactive Materials

Article Title: Enzymatic cross-linking collagen-based bioink for 3D bioprinting of endometrial model with endometrial epithelial organoids

doi: 10.1016/j.bioactmat.2026.10.017

Figure Lengend Snippet: EEO/ESC co-culture in C5H2T1 hydrogel. ( a ) Schematic illustration of native endometrial changes during the menstrual cycle. ( b ) In vitro hormonal treatment strategy used to mimic the menstrual cycle. ( c ) Representative bright-field images showing the growth status of EEO/ESC co-cultures. ( d ) Representative confocal fluorescence images showing the growth status of EEO/ESC co-cultures. PanCK indicates epithelial cells, vimentin indicates ESCs, and Hoechst indicates cell nuclei. ( e ) Gene expression profiles after hormonal treatment ( n = 3 biologically independent donor-derived samples). ( f–i) Representative confocal fluorescence images showing the expression of Ki67, ER, MUC1, and PR after hormonal treatment. ( j–m) Quantification of Ki67, ER, MUC1, and PR expression after hormonal treatment ( n = 5 biologically independent donor-derived samples). Data are presented as mean ± SD. Statistical significance was assessed using one-way repeated-measures ANOVA with Geisser–Greenhouse correction followed by Dunnett's multiple comparisons test. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Commercial ELISA kits for IGFBP1 (E-EL-H0442, Elabscience, China), PRL (E-EL-H0141, Elabscience, China), SPP1 (E-EL-H1347, Elabscience, China), and PAEP (EH1142, FineTest, China) were used to measure their levels, respectively, according to the manufacturers’ protocols.

Techniques: Co-culture Assay, In Vitro, Co-Cultures, Confocal, Fluorescence, Gene Expression, Derivative Assay, Expressing

Journal: Bioactive Materials

Article Title: Enzymatic cross-linking collagen-based bioink for 3D bioprinting of endometrial model with endometrial epithelial organoids

doi: 10.1016/j.bioactmat.2026.10.017

Figure Lengend Snippet: Comparison of the collagen-printed model using C5H2T1 bioink and the Matrigel-cast model. ( a ) Gene expression profiles after hormonal treatment ( n = 3 biologically independent donor-derived samples). Data are presented as mean ± SD. Statistical significance was assessed using two-way repeated-measures ANOVA with Geisser–Greenhouse correction, followed by Dunnett's multiple comparisons test for comparisons with control within each group and Šídák's multiple comparisons test for comparisons between Matrigel and collagen under the same treatment condition. ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. ( b ) UMAP plot showing the distribution of cell subpopulations. ( c ) Bubble plot showing gene expression patterns across cell subpopulations. Bubble size represents the proportion of cells expressing a given gene within each cluster, while the color gradient from blue to red indicates the average expression level from low to high. ( d ) RNA velocity analysis. ( e ) UMAP plot comparing the collagen-printed and Matrigel-cast groups. ( f ) UMAP visualization of DPP4 and KLF5 expression. ( g ) GO enrichment analysis of significantly upregulated genes in SOX9 + _Epi from the collagen-printed group compared with the Matrigel-cast group. ( h ) UMAP visualization of MSLN and WNT7A expression. i ) GO enrichment analysis of significantly downregulated genes in SOX9 + _Epi from the collagen-printed group compared with the Matrigel-cast group.

Article Snippet: Commercial ELISA kits for IGFBP1 (E-EL-H0442, Elabscience, China), PRL (E-EL-H0141, Elabscience, China), SPP1 (E-EL-H1347, Elabscience, China), and PAEP (EH1142, FineTest, China) were used to measure their levels, respectively, according to the manufacturers’ protocols.

Techniques: Comparison, Gene Expression, Derivative Assay, Control, Expressing, Analysis

Journal: Bioactive Materials

Article Title: Enzymatic cross-linking collagen-based bioink for 3D bioprinting of endometrial model with endometrial epithelial organoids

doi: 10.1016/j.bioactmat.2026.10.017

Figure Lengend Snippet: Primer sequences.

Article Snippet: Commercial ELISA kits for IGFBP1 (E-EL-H0442, Elabscience, China), PRL (E-EL-H0141, Elabscience, China), SPP1 (E-EL-H1347, Elabscience, China), and PAEP (EH1142, FineTest, China) were used to measure their levels, respectively, according to the manufacturers’ protocols.

Techniques:

Journal: Matrix biology : journal of the International Society for Matrix Biology

Article Title: Molecular and epigenetic ex vivo profiling of testis cancer-associated fibroblasts and their interaction with germ cell tumor cells and macrophages.

doi: 10.1016/j.matbio.2024.06.001

Figure Lengend Snippet: Fig. 4. (A) Visualization of the RNAseq expression data and (B) LC-MS-based secretome data of IGFBP1 / IGFBP1, LGALS3BP / LGALS3BP, LYVE1 / LYVE1, and PTX3 / PTX3 in CAF populations compared to nFB, and (C) qRT-PCR-based validation of IGFBP1, LGALS3BP, LYVE1, and PTX3 expression in nFB and CAF (SE-CAF = 6, EC- CAF = 3, TER-CAF = 3, nFB = 5). SD is based on biological replicates. GAPDH and ACTB were used as housekeepers and for data normalization. (D) Measurement of LGALS3BP secretion in nFB and CAF via ELISA, dots indicate the biological replicates (n = 3 / subgroup). (E) Cell proliferation assay of TCam-2 (SE) and 2102EP, NCCIT, NT2/D1 (EC) treated daily with recombinant proteins (LGALS3BP, LYVE1, IGFBP1; 10 - 100 ng / mL) over 10 d. SE-related SD is based on technical triplicates; EC-related SD is based on biological triplicates (each in technical triplicates). (F) qRT-PCR analysis of expression of cisplatin resistance-associated factors in TCam-2 (SE) and 2102EP, NCCIT, NT2/D1 (EC) treated daily with recombinant proteins (LGALS3BP, LYVE1, IGFBP1; 10 - 100 ng / mL) over 10 d SE-related SD is based on technical triplicates; EC-related SD is based on biological triplicates (each in technical triplicates). GAPDH and ACTB were used as housekeepers and for data normalization. Dashed lines indicate the threshold of a FC > 1.5 / < -1.5.

Article Snippet: For TCam-2, 1300 cells and for EC cells, 2650 cells per well of a 24- well plate were seeded, treated daily with recombinant protein LGALS3BP (2226-GAB), LYVE1 (2089-LY) or IGFBP1 (871-B1–025) (all R&D Systems) and counted in technical triplicates over 10 d using a Neubauer counting chamber.

Techniques: Expressing, Liquid Chromatography with Mass Spectroscopy, Quantitative RT-PCR, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Proliferation Assay, Recombinant

Journal: International Journal of Molecular Sciences

Article Title: Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding

doi: 10.3390/ijms232314671

Figure Lengend Snippet: Hepatic SCD1 deficiency up-regulates IGFBP1. ( A ) Relative hepatic Igfbp1 mRNA levels in 8–10 weeks old male WT (n = 4) and GKO (n = 6) mice fed HCD for 10 days. ( B ) Relative hepatic Igfbp1 mRNA levels in 8–13 weeks old male LOX (n = 13) and LKO (n = 12) mice fed HCD for 7–10 days. ( C ) Plasma IGFBP1 protein concentration in 8–10 weeks old male LOX (n = 7) and LKO (n = 7) mice fed HCD for 7–10 days. ( D ) Relative IGFBP1 mRNA levels in HepG2 cells treated for 24 h with BSA (n = 10) or BSA plus 4 μM (n = 12) of SCD inhibitor. Values are mean ± SEM; * p < 0.05 vs. WT, LOX, or BSA, *** p < 0.005 vs. WT, LOX or BSA by Student’s two-tailed t -test.

Article Snippet: Lastly, secreted IGFBP1 protein was measured using the Human IGFBP-1 ELISA Kit PicoKineTM (Boster Biological Technology, Pleasanton, CA, USA, Catalog #EK0382).

Techniques: Clinical Proteomics, Protein Concentration, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding

doi: 10.3390/ijms232314671

Figure Lengend Snippet: Oleic acid blunts IGFBP1 up-regulation in vivo and in vitro. ( A ) Relative hepatic Igfbp1 mRNA levels in 12–14 weeks old male LOX and LKO mice fed either HCD supplemented with 15% by weight tristearin (n = 4 LOX and n = 4 LKO) or 15% by weight triolein (n = 5 LOX; 7 LKO) for 10 days. ( B ) Relative hepatic Igfbp1 mRNA levels in 8–10 weeks old male WT (n = 4), GKO (n = 6), and SCD5tg (n = 5) mice fed HCD for 10 days. ( C ) Relative Igfbp1 mRNA level in HepG2 cells treated for 24 h with either BSA (n = 10), BSA plus 4 μM SCD inhibitor (n = 12), BSA plus 4μM SCD inhibitor, and 500 μM oleic acid (n = 12), or BSA plus SCD inhibitor and 500 μM steric acid (n = 12). ( D ) Secreted IGFBP1 protein concentration in HepG2 cells treated for 24 h with either BSA (n = 7), BSA plus 4 μM SCD inhibitor (n = 8), BSA plus 4 μM SCD inhibitor and 500 μM oleic acid (n = 7), or BSA plus SCD inhibitor and 500 μM steric acid (n = 8). Values are mean ± SEM; * p < 0.05 vs. WT, LOX, or BSA; ** p < 0.01 vs. WT, LOX, or BSA; *** p < 0.005 vs. WT, LOX triolein or BSA by Student′s two-tailed t -test.

Article Snippet: Lastly, secreted IGFBP1 protein was measured using the Human IGFBP-1 ELISA Kit PicoKineTM (Boster Biological Technology, Pleasanton, CA, USA, Catalog #EK0382).

Techniques: In Vivo, In Vitro, Protein Concentration, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding

doi: 10.3390/ijms232314671

Figure Lengend Snippet: Inhibition of mTORC1 by rapamycin suppresses IGFBP1 induction. ( A ) Relative hepatic Igfbp1 mRNA levels in 10–12 weeks old male LOX and LKO mice fed HCD diet for 10 days treated daily with either vehicle (n = 3 LOX; 3 LKO) or rapamycin (n = 5 LOX + R; 5 LKO + R). ( B ) Relative Igfbp1 mRNA levels of HepG2 cells treated with either BSA (n = 5), BSA plus 1μM SCD inhibitor (n = 5), or BSA plus 4 μM SCD inhibitor and rapamycin (n = 5). Values are mean ± SEM; * p < 0.05 vs. LOX or BSA; ** p < 0.01 vs. LOX+V or BSA; *** p < 0.005 vs. LOX or BSA by Student′s two-tailed t -test.

Article Snippet: Lastly, secreted IGFBP1 protein was measured using the Human IGFBP-1 ELISA Kit PicoKineTM (Boster Biological Technology, Pleasanton, CA, USA, Catalog #EK0382).

Techniques: Inhibition, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding

doi: 10.3390/ijms232314671

Figure Lengend Snippet: IGFBP1 is regulated by SCD1 through the mTORC1-FGF21 axis. ( A ) Relative hepatic Scd1 and Fgf21 mRNA levels in LOX/LOX (n = 6, 3 male; 3 female) and DLKO (n = 6, 3 male; 3 female) mice fed HCD for 10 days. ( B ) Relative hepatic Igfbp1 mRNA level in LOX/LOX (n = 6, 3 male; 3 female) and DLKO mice fed HCD (n = 6, 3 male; 3 female) for 10 days. ( C ) Relative Fgf21 mRNA level in HepG2 cells treated with BSA (n = 6), BSA plus 1 μM SCD inhibitor (n = 6), BSA plus 1 μM SCD inhibitor and 100 μM oleic acid (n = 5), BSA plus 1 μM SCD inhibitor and 100 μM steric acid (n = 5), BSA plus 1 μM SCD inhibitor and rapamycin (n = 5). ( D ) Relative FGF21 mRNA level in HepG2 cells treated with control lentiviral particles (n = 6), control lentiviral particles plus SCD inhibitor (n = 6), or shFGF21 lentiviral particles plus SCD inhibitor (n = 6). ( E ) Relative IGFBP1 mRNA level in HepG2 cells treated with control lentiviral particles (n = 6), control lentiviral particles plus SCD inhibitor (n = 5), or shFGF21 lentiviral particles plus SCD inhibitor (n = 5). Values are mean ± SEM; ns = not significant; * p < 0.05 vs. LOX/LOX, BSA, or control; ** p < 0.01 vs. LOX/LOX, BSA, or control; *** p < 0.005 vs. LOX/LOX, BSA, or control by Student′s two-tailed t -test.

Article Snippet: Lastly, secreted IGFBP1 protein was measured using the Human IGFBP-1 ELISA Kit PicoKineTM (Boster Biological Technology, Pleasanton, CA, USA, Catalog #EK0382).

Techniques: Control, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Hepatic Oleate Regulates Insulin-like Growth Factor-Binding Protein 1 Partially through the mTORC1-FGF21 Axis during High-Carbohydrate Feeding

doi: 10.3390/ijms232314671

Figure Lengend Snippet: Proposed model of IGFBP1 induction through the mTORC1-FGF21 axis.

Article Snippet: Lastly, secreted IGFBP1 protein was measured using the Human IGFBP-1 ELISA Kit PicoKineTM (Boster Biological Technology, Pleasanton, CA, USA, Catalog #EK0382).

Techniques: